HEK293 Cells: SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD
(682-GSAS-685, K986P/V987P)-His Recombinant Protein
(682-GSAS-685, K986P/V987P)-His Recombinant Protein
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Protein Construction:
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein
(S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(682-GSAS-685, K986P/V987P)) was expressed with the phage trimerization foldon domain and a polyhistidine tag at the C-terminus. Source: 2019-nCoV
Expression Host: HEK293 Cells
Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Molecular Mass:
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2
ECD (Met1-Pro1213(682-GSAS-685, K986P/V987P)-His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
Formulation:
Storage buffer from sterile PBS, pH 7.4
Normally 10% glycerol, mannitol and 0.01% Tween 80 are added as protectants before store at - 20℃ to -80℃. Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag at
1 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 Recombinant Protein, the EC50 of SARS-CoV-2 (2019-nCoV)
Spike S1+S2 ECD (Met1-Pro1213(682-GSAS-685, K986P/V987P)-His tag) recombinant Protein is 50-100 ng/mL
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Molecular Mass:
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2
ECD (Met1-Pro1213(682-GSAS-685, K986P/V987P)-His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
Formulation:
Storage buffer from sterile PBS, pH 7.4
Normally 10% glycerol, mannitol and 0.01% Tween 80 are added as protectants before store at - 20℃ to -80℃. Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Measured by its binding ability in a functional ELISA.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag at
1 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 Recombinant Protein, the EC50 of SARS-CoV-2 (2019-nCoV)
Spike S1+S2 ECD (Met1-Pro1213(682-GSAS-685, K986P/V987P)-His tag) recombinant Protein is 50-100 ng/mL