AM-50 Heparin resin/ Heparin 樹脂

EBL AM-50 Heparin resin 是一種成熟的親和性樹脂,設計用於捕獲或居中參與純化步驟。該介質被廣泛應用於市售血漿蛋白的工業純化,包含工業上的抗凝血酶III (antithrombin III)、凝血因子和其他血漿蛋白的純化。偶聯化學 (Coupling chemistry) 產生化學穩定的配體連接,AM-50 Heparin resin 由單一分散的聚合beads 組成,通過使用化學穩定且定向的耦合劑將配體分子偶聯到基質上,從而提供高結合能力。介質的穩定性僅由 heparin ligand 決定。此產品提供從實驗室規模到工業應用的利用。

以下為常使用Heparin 介質純化的蛋白質:
mast cell proteases, lipoprotein lipase, coagulation enzymes, elastases, extracellular superoxide dismutase, serine protease inhibitors, such as AT3, heparin cofactor 2 and protease nexin, which inactivates serine proteases in the extracellular matrix. Other heparin binding proteins in the extracellular matrix are vitronectin, fibronectin, thrombospondin, laminin, collagen and Growth factors such as fibroblast growth factor (FGF), Schwann cell growth factor, retinal survival factor and homopoietic growth factor

產品規格:
Particle Size (μm)
50
Pore Size (Å)
800
Ligand Density
about 5 mg/mL heparin of porcine origin
Target Protein Binding Capacity (g/ml)
2 mg antithrombin III
Bead Matrix
Polyacrylate
Recommended Flow Rate (cm/h)
200-400
pH Stability
4-13
Pressure (MPa)
10
Chemical stability
0.1 M NaOH (1 week at 20°C)
0.05 M sodium acetate
4 M NaCl, pH 4.0
8 M urea
6 M guanidine hydrochloride
Storage buffer
0.05 M sodium acetate containing 20% ethanol
Storage temperature
+4–8 °C

 
訂購資訊:

Catalog Number: amm-5005020, 20 ml
Catalog Number: amm-5005100, 100 ml