Mini Spin Column 主要應用於快速製備蛋白質樣品。
專為小量樣品而開發，快速且易於使用。 可以用來篩選純化條件。
產品優點:
專為小量樣品而開發，快速且易於使用。 可以用來篩選純化條件。
產品優點:
● 配合離心機可在30分鐘內完成12個樣品製備
● 簡化操作、 無需昂貴的儀器
● 10 um孔徑適合所有親和性介質
Quick protocol：
1. Mix the sample solution comprising the target proteins (50 ul-2 ml) with the proper volume of media (100-200 ul) well for 5-10 minutes.
2. Set the tube containing the mixture above vertical so that the media precipitate to the bottom of the tube.
3. Remove most of the supernatant, leaving the mixture to be around 800 ul.
4. Mix and transfer the samples to the columns with the pipetman.
5 The mixtures are centrifuged with 3,000-5,000 rpm for 10-15 seconds. Collect the flow sup.
6. 800 ul wash buffer is added to mixtures. The mixtures are centrifuged with 3,000-5,000 rpm for 10-15 seconds. Collect the flow sup.
7. Repeat step 6.
8. Transfer the mixtures to new 1.5 ml tubes, add 50-100 ul elution buffer to them, mix them well and allow them to be stable for 1 minute.
9. The mixtures are centrifuged with 3,000-5,000 rpm for 10-15 seconds. Collect the eluants. Analyze them and the flow sup. by SDS-PAGE or measuring the OD.
NOTE:
● For high performance samples or minor samples, mix with the media in the columns directly (the media must be balanced by wash buffer in advance).
● Repeat the eluting process 1-2 times during elution. Or perform elution with different concentration based on the need of your experiment.
訂購資訊:
訂購資訊:
Catalog Number: spc-00800, 800 ul, 50/pk